https://cfy2yue.github.io/publication_types/article/ArticleHugoBlox Kit (https://hugoblox.com)en-usTue, 04 Mar 2025 00:00:00 +0000https://cfy2yue.github.io/media/icon_hu_1c0e9cb08cfb822a.pnghttps://cfy2yue.github.io/publication_types/article/https://cfy2yue.github.io/publications/spcas9-gear-wedge/Tue, 04 Mar 2025 00:00:00 +0000https://cfy2yue.github.io/publications/spcas9-gear-wedge/<p><strong>Citation.</strong> Shaoyong Lu, Xinyi Li, Jiacheng Wei, <strong>Feiying Chen</strong>, Mingyu Li, Ning Liu, and Jian Zhang. Untangling the molecular mechanism of SpCas9 catalytic activation: A gear-and-wedge fitting model. Under review.</p> <p>This manuscript links atomistic simulation to biochemical and cellular validation, showing how linker remodeling and REC2-domain motion coordinate the large HNH-domain displacement required for SpCas9 activation.</p>